Beta-glucans Blocker mo te Aukati i te Ara Beta Glucan
Beta-glucans Blocker mo te Aukati i te Ara Beta Glucan
1. Nga korero hua
E rua nga ara i roto i te limulus amoebocyte lysate lal reagent, ko te huarahi tauwehe C he mea motuhake ki te endotoxin me te huarahi tauwehe G he mea motuhake ki te (1,3)- β-D-Glucans.Mena kei roto i te tauira whakamatautau te β-1,3-Glucans, ka raru te whakamatautau limulus (Endotoxin test).Ka aukati a β-G-Blocker i te tauhohenga o te LAL ki te β-1,3-Glucans, me te whakanui ake i te motuhake endotoxin ki te whakamatautau LAL.Mena kei roto i nga tauira whakamatautau te β-1,3-Glucan, penei i te cellulose, te whakamahibeta-glucans aukatihe whakaaro pai ki te aukati i te pokanoa β-1,3-Glucan ki te endotoxin me te tauhohenga reagent LAL.
2. Tawhā hua
Te taumata Endotoxin iti iho i te 0.005EU/ml
3. Nga ahuatanga hua me te tono
Whakakapihia te LAL Reagent Water ki te whakahou i te reagent LAL, ka aukati i te beta-glucan ka takawaenga i te ara take G me te whakarite ko te reagent LAL ka tauhohe ki te endotoxin anake.Mo nga tauira whakamatautau kei a (1,3)- β-D-Glucan parahanga.
| Putumōhio No. | Whakaahuatanga | Tuhipoka | Mōkī |
| BH10 | 50mM Tris parepare, pH7.0, 10ml/ ipu | Ka whakamahia ki te waimeha i nga tauira tino waikawa, taketake ranei. | 10 ipu / kete |
| BH50 | 50mM Tris parepare, pH7.0, 50ml/ ipu | Ka whakamahia ki te waimeha i nga tauira tino waikawa, taketake ranei. | 10 ipu / kete |
| BY10 | 10mM Pūrei konupora, 10ml/ ipu | I whakamahia ki te hinga i te paanga chelation. | 10 ipu / kete |
| BT10 | β-glucans paraka, 10ml/ ipu | Ka whakamahia hei aukati i te pokanoa β-glucan i roto i nga tauhohenga endotoxin Amebocyte Lysate. | 10 ipu / kete |
| PBS50 | PBS aukati endotoxin-kore, 50ml/ ipu | Whakamahia hei horoi i nga ipu tauira, hei whakatika ranei i te pH | 10 ipu / kete |
| PBS500 | PBS aukati endotoxin-kore, 500ml/ ipu | 1 ipu |
Tikanga Hua
Ko te tairongo o te Lyophilized Amebocyte Lysate me te kaha o te Endotoxin Paerewa Whakahaere ka whakamatauria ki te USP Reference Standard Endotoxin.Ka tae mai nga kete reagent Lyophilized Amebocyte Lysate me nga tohutohu hua, Tiwhikete Tiwhikete.
Waiho o Karere
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