Amebocyte Lysate Whakamātau-maha Vial G52

Ko te raupapa Bioendo G52 te nuinga o te whakamahi i roto i nga mahi whakamatautau owhakamātautau endotoxin huakitahei tikanga Bioassay.

1. Nga korero hua

Ko te tikanga o te Gel Clot Lyophilized Amebocyte Lysate Multi-test Vial ko te Lyophilized Amebocyte Lysate reagent e whiriwhiri ana, e whakamahi ana i te tikanga ki te kimi i te endotoxin, pyrogen ranei.

I te mea ko te tikanga whanui, he maamaa te whakamatautau gel-clot mo te endotoxin kaore e hiahiatia he taputapu motuhake me te utu nui.Ka whakaratohia e Bioendo te Gel Clot Lyophilized Amebocyte Lysate - LAL reagent i roto i te 5.2ml mo ia oko.

2. Tawhā Hua

Awhe tairongo: 0.03EU/ml, 0.06EU/ml, 0.125EU/ml, 0.25EU/ml, 0.5 EU/ml

3. Tono Hua

Whakamutunga hua endotoxin (pyrogen) tohu, wai mo te werohangawhakamātautau endotoxin, rauemi matawhakamatautau endotoxinte aro turuki i te taumata endotoxin i te wa o te mahi whakangao mo nga kamupene rongoa, kaihanga taputapu rongoa ranei.

Tuhipoka:

Ko te Lyophilized Amebocyte Lysate (LAL reagent) i hangaia e Bioendo he mea hanga mai i te lysate o nga amebocytes (nga pūtau toto ma) mai i te papaka hoiho.

Ko tenei reagent ahurei kua waiho hei taputapu nui i roto i nga umanga rongoa me nga taputapu rongoa mo te rapu i nga endotoxins huakita.Kei roto i nga amebocytes o te papaka hoiho he matū e kiia nei ko Lyophilized Amebocyte Lysate, e tauhohe ana ki nga endotoxins huakita ma te hanga i te ahua o te reera.Ko tenei tauhohenga te putake mo te whakamatautau LAL, i whakamahia hei whakarite i te haumaru o nga taputapu rongoa, raau taero me etahi atu hua ka pa ki te tinana o te tangata.

Ko te whakamahinga o te reagent LAL kua huri te tukanga ote kitenga endotoxini roto i te mara hauora atu i te whakamatautau Rabit.Ko ona ahuatanga me te motuhake ka waiho hei waahanga nui i roto i te mana kounga me te whakapumautanga haumaru o nga rongoa, koiora me nga taputapu rongoa.Ko te whakamatautau LAL he tikanga tere me te pono mote kitenga endotoxin, whakarato hua i roto i te iti rite 60 meneti.Ma tenei mahi ka taea te whakatau tere me te tika mo te tukunga o nga hua, i te mutunga ka piki ake te haumaru me te whaihua o nga rongoa rongoa me nga taputapu.

Ko te Lyophilized Amebocyte Lysate (LAL reagent) a Bioendo ka hangaia i raro i nga paerewa kounga tino hei whakarite i tona whaihua me te pono.E whakatapua ana te kamupene ki te whakamahi i nga tikanga pumau i roto i te hauhake i nga papaka hu hoiho hei whakaiti i nga paanga kino ki o raatau taupori.Ma te aro nui ki te oranga o enei mea hanga, ka whakarite a Bioendo i te tuku tonu o tenei rauemi tino nui mo te hanga o nga reagents LAL.I tua atu, ko nga mahi rangahau me te whakawhanaketanga kei te arotahi ki te whakapai ake i te mahi me te whai kiko oLAL whakamatautau endotoxin, te whakatairanga ake i a raatau mahi i roto i nga umanga rongoa me te rongoa.

Ko te tikanga o te kaeraKo te whakamatautau LAL, ko te reagent lysate kua whakahouhia kia 50 nga whakamatautau mo ia ipu:

huru hua:

Ko te Lyophilized Amebocyte Lysate - LAL reagent sensitivity me te Mana Paerewa Endotoxin kaha ka whakamatauria ki te USP Reference Standard Endotoxin.Ka tae mai nga kete reagent Lyophilized Amebocyte me nga tohutohu hua, Tiwhikete Tiwhikete, MSDS.

He aha te rereketanga i waenga i te ipu whakamatautau kotahi a Bioendo me te ipu whakamatautau maha?

● Whakamātautau kotahi: whakahouhia te kotahiwhakamātautau limulus lysateka karanga raneilimulus amebocytena te wai BET i roto i te ipu karaihe, i te ampoule karaihe ranei.

● Whakamātautau-maha: whakahiatotia te reagent lysate ki te wai BET, katahi ka tapirihia te nui o te reagent lysate whai muri i te COA ki te ngongo tauhohenga, ki te pereti poka hei whakamahi.Karekau he rereketanga o te tauira i mua i te tukatuka;i runga i te nui o te whakamatautau i whakamahia, ko te rahi o te tauira e whakamahia ana mo te whakamatautau kotahi he nui ake i te rahinga tauira e whakamahia ana mo nga whakamatautau maha.

He aha te take i motuhake ai te kete G52 mo te maha o nga tauira papatipu?

1. Whakamātautau maha tauhohenga LAL mo te rapunga endotoxin i roto i nga tono o nga tikanga whakahaere whakamatautau LAL tauira papatipu.

2. Ko te raupapa G52 o te kaakaa o te kaareti endotoxin te whakamatautau maha ipu karaihe whakamatautau kaore e hiahiatia he kaipanui microplate matatau.I roto i te whakamatautau LAL, he mea watea te tikanga mo te whakamaarama ma te kaukau wai, te incubator wera maroke ranei.

3. Ko te kounga teitei o te ngongo kore utu endotoxin (<0.005EU/ml) me te Kounga teitei o nga tohutohu kore utu pyrogen (<0.005EU/ml) hei kai whakapumau hei whakarite i te hua tika.

4. Ki te whiriwhiri i te pouaka whakamatautau LAL kotahi a Bioendo, te ipu whakamatautau LAL maha ranei ma te rahinga tauira, ko te whaainga koKo te whakamatautau LAL mo te pyrogensrapunga.

Nga hua e pa ana ki te whakamatautau endotoxin:

Te wai mo te Whakamatau Endotoxins Bacterial (BET), Tohutohu TRW50, TRW100 ranei

Endotoxin ngongo karaihe kore ( ngongo waimeha ), Tohutohu T1310018 me T107540

Nga tohutohu kore utu a Pyrogen, Tutohu PT25096 PT100096 ranei

Pipettor, Tohutohu PSB0220

Rack Tube Test

Taputapu Incubation (Water Bath or Dry Heat Incubator ), ki te tūtohu Bioendo Dry Heat Incubator TAL-M2 he 60 rua kotahi modular.

Vortex Mixter, Tohutohu VXH.

Whakahaere Paerewa Endotoxin, CSE10V.